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Products for cheesemaking
.Starters
Mould Spores
Lipase
Calcium Solution
Cheese Wax
Thermometer
Cheese Cloth
Rennet

The following information was extracted from the book "HOME CHEESEMAKING" by Neil and Carole Willman

Starters:
What is a starter?
A starter is not rennet or junket but a specially selected bacteria. Starters are chosen because they possess certain characteristics which are of value to cheesemaking. These characteristics can include such things as acid production, enzyme production, flavour component production or gas production (carbon dioxide). Some starters have one of these characteristics, while others have more than one. Starters are very tiny. In one millilitre of liquid starter there are about 200,000,000 (two hundred million) living starter organisms.
Why do we use starters?
Starters are mainly used for acid production. Many years ago naturally occurring bacteria in the milk were used, however their numbers are inconsistent and many of the bacteria in raw milk are spoilage organisms. The quality of cheeses made from raw milk will be quite variable. Pasteurisation kills many of the desirable bacteria in raw milk and hence the need to add starters to replace them. Acid production is necessary in the making of cheese, as the acid helps the curd to form and shrink, as well as preventing the growth of spoilage bacteria and determining the characteristics of the cheese. 

Mould Spores:
Mould spores are sometimes used in the manufacture of cheese. These are available in two categories; Blue mould spores (Penicillium roqueforti) and White mould spores (Penicillium candidum). Blue mould spores are used for making Blue Vein, Roquefort, Stilton and Gorgonzola cheeses. White mould spores are used for making Camembert and Brie.
Others Other organisms can be used in the making of other cheese types. These include a bacteria (Propionibacterium shermanii) added to the milk to provide extra gas production to form the eyes in Swiss cheese, and a bacterial culture used for rubbing on the surface of cheeses such as Tilsit and Havarti(Brevibacterium linens). 

Lipase:
Lipase is an enzyme (protein material) which has the function of breaking down the fats of the milk or cheese. Cheeses which use lipase during their manufacture are Fetta, Romano, Pecorino, Parmesan, and many others. 

Calcium Solution:
Calcium chloride solution is used to assist cheesemakers when milk is calcium deficient. It assists the milk coagulation process for rennet set cheeses.

Cheese Wax:
Cheesewax provides a suitable moisture barrier on the cheese to prevent rapid drying. They are a mixture of microcrystalline wax and parrafin. Hard cheeses that are going to be stored for more than one month will dry out and crack if not protected. They may also become mouldy and inedible. These cheeses may be waxed before maturing to prevent them from drying out. The waxing will be more successful if you apply a plastic cheese coating to the cheese first. This gives the wax a fat free and moisture free surface on which to adhere. Better results are obtained when a plastic cheese coating is applied first. 

Plastic Cheese Coating:
The plastic coating is a PVA emulsion specially designed for use on cheese. Two coats of plastic cheese coating are normally applied by brushing with a small paint brush, or rubbing the coating on with a coarse sponge.

Thermometer:
It is important to have a good accurate thermometer for cheese and yoghurt making. A thermometer with a scale of -10 to 110°C with graduations at 1°C intervals is usually satisfactory

Cheese Cloth:
The cheese cloth is also known as cheese bandage. It is applied to rinded cheese to assist in the development of a good rind. The cloth stays on the cheese during storage. 

Rennet:
Rennet may be from animal or vegetative sources. Traditionally calf rennet was used, but vegetative alternatives are now common. Vegetative rennets are made from fungal origin with Mucor mehei being the most commonly used.

Genetic engineering techniques some 'vegetarian' cheeses made using chymosin by genetically engineered microorganisms. The genetic material (DNA) which enables chymosin replication is placed into a microorganism which is then cultured producing chymosin. This chymosin is identical to that found in calf rennet. The organisms used to produce GE Chymosin are either a yeast, Kluyveromyces lactis, or a bacteria Escherichia coli.

Rennet is an enzyme which reacts with the protein in the milk to coagulate the milk. It is therefore added to the milk to turn it from the liquid state to a solid state. The active enzyme in calf rennet is known as Chymosin.

When using rennet always dilute it in 10 times its volume of cool boiled water. If you don't do this you will find that your rennet has started working before you have mixed it in properly, giving a very uneven curd. Be sure that the water you are using is not hot or it will adversely affect the activity of you rennet. Do not sanitise rennet measuring utensils with sodium hypochlorite. Sodium hypochlorite will adversely affect the activity of your rennet. You will need to rinse these utensils with boiling water.

Hard cheeses cannot be made without rennet. Hard cheeses claiming to be rennet free are more correctly 'animal rennet' free. These cheeses are made from microbially produced chymosin, which is classed as fermented or vegetarian rennet.

The above information was extracted from the book "HOME CHEESEMAKING" by Neil and Carole WillmanBack to top of this page - Back to CHEESEWORKSHome Page


Cheese and Rennet
an Information Sheet from the Vegetarian Society (UK)


What is Cheese? What is Rennet?

Cheese is made by coagulating milk to give curds which are then separated from the liquid, whey, after which they can be processed and matured to produce a wide variety of cheeses. Milk is coagulated by the addition of rennet. The active ingredient of rennet is the enzyme, chymosin (also known as rennin). The usual source of rennet is the stomach of slaughtered newly-born calves. Vegetarian cheeses are manufactured using rennet from either fungal or bacterial sources. Advances in genetic engineering processes means they may now also be made using chymosin produced by genetically altered microorganisms. 

Cheese Manufacturing Process

The exact processes in the making of cheese varies between different varieties. However, all cheeses are made by essentially the same method. Initially, the milk is usually pasteurised by heating at 72 degrees C for 15 seconds to destroy potentially harmful bacteria. The milk is then cooled to around 30 degrees C and a starter culture of lactic acid bacteria is added to help souring. These convert lactose into lactic acid and help in the coagulation process. In addition, they also have a beneficial effect on the eventual quality, taste and consistency of the cheese. Some cheeses are coagulated entirely by lactic acid bacteria and are known as lactic curd or acid-curd cheeses. However, some cheeses sold as lactic-curd cheese may have had rennet added. 

The next stage is the addition of rennet, containing the enzyme chymosin. Rennet is usually sourced from the abomasum (fourth stomach) of newly-born calves. Here, chymosin aids the digestion and absorption of milk. Adult cows do not have this enzyme. Chymosin is extracted by washing and drying the stomach lining, which is then cut into small pieces and macerated in a solution of boric acid or brine at 30 degrees C for 4-5 days. Pepsin may sometimes be used instead of chymosin. This is usually derived from the abomasum of grown calves or heifers, or less commonly pigs. Pepsin may be mixed with calf rennin. Rennet coagulates the milk, separating it into curds and whey. This is called curdling. Chymosin breaks down the milk protein casein to paracasein which combines with calcium to form calcium paracaseinate, which separates out. Milk fat and some water also becomes incorporated into this mass, forming curds. The remaining liquid is the whey. The strength of different rennets can vary, though usual strength varies between 1:10 000 and 1:15 000 ie one part rennin can coagulate 10 - 15 000 parts milk. 

Other substances may also be added during the cheese making process. Calcium chloride is added to improve the curdling process, and potassium nitrate is added to inhibit contaminating bacteria. Dyes (eg annatto, carotene), Penicillium roquefortii mould spores to promote blue veining, or propionic acid bacteria to encourage hole formation may be added. 

Following curdling, the curds are cut and drained. The size of the cut and the methods used vary for different cheese varieties. For soft cheeses, the curds are sparingly cut and allowed to drain naturally. For hard cheeses, the curds are heated and more whey is drained off. The curds are then cut into small pieces, placed in vats and pressed. 

After pressing, the curds may be treated in a number of ways. They may be moulded into different shapes, soaked in a saltwater solution, be sprayed with mould forming spores or bacteria, washed in alcohol, or covered in herbs. 

The final stage is ripening, or maturation. This can vary in length from 4 weeks to 2-3 years, depending on the type of cheese. During ripening flavours develop, the cheese becomes firmer and drier, and special characteristics such as holes, blue veining and crust formation occurs. 

Vegetarian Cheeses

Vegetarian cheeses are made with rennets of non-animal origin In the past, fig leaves, melon, wild thistle and safflower have all supplied plant rennets for cheese making. However, most widely available vegetarian cheeses are made using rennet produced by fermentation of the fungus Mucor miehei. Vegetarian cheese may also be made using a rennet from the bacteria Bacillus subtilis or Bacillus prodigiosum. 

Advances in genetic engineering techniques mean that some 'vegetarian' cheeses may now be made using chymosin produced by genetically engineered microorganisms. The genetic material (DNA) which encodes for chymosin is introduced into a microorganism which can then be cultured to produce commercial quantities of chymosin. This is done by extracting genetic material from calf stomach cells which acts as a template for producing the chymosin encoding DNA. This can then be introduced into the microorganism. Once the genetic material is introduced there is no further need for calf cells. Alternatively, the chymosin encoding DNA can be biosynthesised in the laboratory without the use of calf cells. 

The chymosin produced is identical to that produced by calf stomach cells. The development of genetically engineered chymosin has been encouraged by shortages and fluctuations in cost of rennet from calves. It's manufacturers claim that genetically engineered chymosin will end the cheese making industry's reliance on the slaughter of calves. 

Chymosin encoding DNA has been introduced into three different microorganisms. These are the yeast Kluyveromyces lactis, the fungus Aspergillus niger var awamori, and a strain of the bacteria Escherichia coli. All of these have now been approved and cleared for use by the Ministry of Agriculture, Fisheries & Food (UK). There is no legal requirement for manufacturers to state whether a genetically engineered rennet has been used in the cheese making process. 

Vegetarian cheeses are widely available in supermarkets and health food stores. A wide variety of cheeses are now made with nonanimal rennet and labelled as suitable for vegetarians. No particular type of cheese is exclusively vegetarian. Soft cheeses are as likely to be non-vegetarian as hard cheeses. A list of cheeses suitable for vegetarians is available from The Vegetarian Society. 

Types of Cheese

The type of cheese produced depends on the milk used and the cheese making process. The milk used may be full fat, semi-skimmed or fully skimmed, this affecting the fat content of the cheese. It may be pasteurised or unpasteurised. Milk from different animals and different breeds is important in determining the final flavour. As well as cow's milk, cheese may be made from sheep or goat's milk. 

Soft cheeses may be fresh or ripened. Fresh cheeses include quark, cottage cheese and cream cheese. Ripened soft cheeses include Brie and Camembert. Semi-soft cheeses include Stilton, Wensleydale and Gorgonzola. Hard cheeses include Cheddar, Cheshire, and Gruyere. Parmesan is a strongly pressed, very hard, dry cheese ripened for 2 - 3 years and then grated. Whey cheeses such as Ricotta are made as a by-product of other cheeses from the whey removed during pressing. Processed cheeses are either made with trimmings that are left over from the manufacture of other cheeses, or from dried milk powder. Flavourings, colourings and other additives are used. 

Nutritional Aspects

Cheese is a good source of protein, calcium, zinc, and vitamin B12. However, full fat cheese is a major source of saturated fat which can lead to raised serum cholesterol levels. Also, it contains no carbohydrate or fibre, and is a very poor source of iron. Vegetarians, particularly new vegetarians, should be wary of too high a consumption of cheese. 
This article is copyright to the Vegetarian Society (UK), but may be freely copied provided it is kept intact, not altered and these lines are included. 

Originally posted in the Usenet newsgroup rec.food.veg by John Davis. 

Converted to HTML by: Donald Graft 

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Copyright © 2000-2008  Carole Willman. Last modified January, 2008.